I bet its the safety clutch. First thing is to make sure the safety clutch hasn't been tripped. There is a little silver button on the bed of your machine. Press this down and rotate the hand wheel back wards. You will feel the button engage at some point ant the machine stop. Continue to push backwards with a fair amount of pressure. If you feel something click back in to place then the clutch is reset and that was your problem. If it doesn't click the clutch is already good. If it just keeps spinning backwards with out locking then there is a little part on the clutch that is broken and needs to be replaced.
I'm no expert on sewing machine repair. I usually take it to get serviced if I have a problem. But if your interested it doing it yourself here is the manual and service manual for the JUKI DNU 1541s. In here it will shows how to adjust all the settings like hook timing.
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Let us know what you find out!